Tuesday, 18 September 2007

Laboratory. *18SG*

Sadly I do not have any pictures of my laboratory mouse.

YES we used mice in our lab today. The mouse was so small and it was like span only half of my palm. It was a dead mouse. It looked so cute and small and it was white in colour. If it is still alive, I would still have killed it :P keke..I am a heartless person and I am sure those who knows me will support me :P

The following descriptions are not for the faint hearted and the person who reads this must imagine to understand better as I do not have pictures of my mouse. Be warned :P

First and foremost, we pulled the skin with a forcep and made a small cut with the scissors. Then we pulled the skin open revealing the peritoneum enclosed by membrane. As we only need the hind limbs, we pulled the skin of the limbs further and we cut off the bone near to the pelvic.

Then we took the limb and pull the skin further down towards the paw and we cut them off as well. After that, we used the scissors and cut off the tissues and muscles as we only need the bones.

After some thorough cleaning, the bones can be seen; femur, tibia and fibula. We pulled the tibia and fibula away from the femur and we cut off the fibula as the bone was too small and too thin to do anything with it. Later we took a needle and a syringe filled with media, poked into the hole in the bones and flush out the bone marrow. This was the only thing we need to analyse and the rest of the mouse can be discarded.

As pitiful as it was, Ann did not plan to leave it to rest in peace. She practically cut off everything revealing the heart, the lungs, the liver, the kidneys, the intestines, etc :P WHat a horrible thing to do to a dead mouse :P hehe

After all those 'dissection' the whole mouse looked like some pile of red thing..Imagine you tried chewing some food before realising it sucks and then you spit them out. It looked just like that :P

We threw the mouse away, counted the cells using haemocytometer and the all expensive advance microscope and we were done. We were the first group among all to finish :) My group consisted of Ann, Wai Yi, Noni and Me.

Our mouse musta been an active one as it has about 20mil cells in total, from only the femur and tibia :P

SO lets have a moment of silence for the mouse :)

4 comments:

Jaded said...

Poor poor mouse.

Evil evil Jiann Wei.

Jiann said...

Me no evil..It is for greater good :) though the greater good is only teaching us how to extract dendritic cells which in other words we only needed the limbs..keke

SilverIsle said...

Woah. Very fun wei. Please tell me no frogs will do. =P

Shu Lin said...

OMG... kesiannya... why la use white mouse? use roaches or something else la... evil!!!

=P